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The user is able to define a hit list based on the dataset of a primary or pilot screen using user-defined thresholds. A choice of negative (less than) and positive (Greater than) thresholds are available (Figure 1, Arrow A). Alternatively the user can choose to produce a list of the top n or bottom n hits (for example, the top 100 and bottom 100 hits).
In order to define a hit list, the user must select the screen they intend to use, as well as the data measurement they wish to apply the threshold to (Figure 1, Arrow B).
Each time an input is changed, the user must click the "Refresh Hit List" button to update the results (Figure 1, Arrow C).
Using this hit list generation, it is possible to see a summary of the hit list produced using the Hit List Summary tab. A summary of where controls fall in the hit list (Figure 1, Arrow D, as well as the distribution of hits according to plate (Figure 1, Arrow E) and well position is shown (Figure 1, Arrow F). This can help identify whether the hit list is biased towards a certain plate, or well position and can indicate a more sophisticated approach may be necessary to define hits. If this is the case, please contact the HTS lab to discuss what you would like to do.
The user can hover over graphs to display information about each data point (Figure 1, Arrow G).
Tables displaying the selected hits are shown in the "Hits" tab. These tables are sortable, each column header can be selected and the hits sorted appropriately. This data can be downloaded as an Microsoft Excel file using the "Download" tab.
All hit lists that are generated using these tools can be downloaded using the "Download" tab.
Data is downloaded as an Microsoft Excel file (.xls). If this is not required, please feel free to contact the HTS lab for plain text versions.