Please reference the following paper if you use this database for your work:

HTS-DB: an online resource to publish and query data from functional genomics high-throughput siRNA screening projects.
Saunders et al., Database 2013.

Francis Crick Insitute siRNA screening publications (most recent first)

  • MDH1 and MPP7 Regulate Autophagy in Pancreatic Ductal Adenocarcinoma.
    New M, Van Acker T, Sakamaki JI, Jiang M, Saunders RE, Long J, Wang VM, Behrens A, Cerveira J, Sudhakar P, Korcsmaros T, Jefferies HBJ, Ryan KM, Howell M, Tooze SA.
    Cancer Res. 79:1884-1898
  • Defining host-pathogen interactions employing an artificial intelligence workflow.
    Fisch D, Yakimovich A, Clough B, Wright J, Bunyan M, Howell M, Mercer J, Frickel E.
    Elife. 2019 Feb 12;8
  • Identification and Validation of Novel Autophagy Regulators Using an Endogenous Readout siGENOME Screen.
    New M, Van Acker T, Jiang M, Saunders R, Long JS, Sakamaki JI, Ryan KM, Howell M, Tooze SA.
    Methods Mol Biol 1880:359-374.
  • A genome-wide RNAi screen identifies the SMC5/6 complex as a non-redundant regulator of a Topo2a-dependent G2 arrest.
    Deiss K, Lockwood N, Howell M, Segeren HA, Saunders RE, Chakravarty P, Soliman TN, Martini S, Rocha N, Semple R, Zalmas LP, Parker PJ.
    Nucleic Acids Res. 47:2906-2921
  • The Dynamics of TGF-β Signaling Are Dictated by Receptor Trafficking via the ESCRT Machinery.
    Miller DSJ, Bloxham RD, Jiang M, Gori I, Saunders RE, Das D, Chakravarty P, Howell M, Hill CS.
    Cell Rep 25:1841-1855
  • Quantitative Analysis Reveals that Actin and Src-Family Kinases Regulate Nuclear YAP1 and Its Export.
    Ege N, Dowbaj AM, Jiang M, Howell M, Hooper S, Foster C, Jenkins RP, Sahai E.
    Cell Syst. 6:692-708.e13
  • The glutathione redox system is essential to prevent ferroptosis caused by impaired lipid metabolism in clear cell renal cell carcinoma.
    Miess H, Dankworth B, Gouw AM, Rosenfeldt M, Schmitz W, Jiang M, Saunders B, Howell M, Downward J, Felsher DW, Peck B, Schulze A.
    Oncogene 37:5435-5450
  • Actomyosin drives cancer cell nuclear dysmorphia and threatens genome stability.
    Takaki T, Montagner M, Serres MP, Le Berre M, Russell M, Collinson L, Szuhai K, Howell M, Boulton SJ, Sahai E, Petronczki M.
    Nat Commun. 8:16013
  • Cytoplasmic ATR Activation Promotes Vaccinia Virus Genome Replication.
    Postigo A, Ramsden AE, Howell M, Way M.
    Cell Rep 19:1022-1032
  • UV Irradiation Induces a Non-coding RNA that Functionally Opposes the Protein Encoded by the Same Gene.
    Williamson L, Saponaro M, Boeing S, East P, Mitter R, Kantidakis T, Kelly GP, Lobley A, Walker J, Spencer-Dene B, Howell M, Stewart A, Svejstrup JQ.
    Cell 168:843-855
  • APC/C Dysfunction Limits Excessive Cancer Chromosomal Instability.
    Sansregret L, Patterson JO, Dewhurst S, López-García C, Koch A, McGranahan N, Chao WCH, Barry DJ, Rowan A, Instrell R, Horswell S, Way M, Howell M, Singleton MR, Medema RH, Nurse P, Petronczki M Swanton C
  • Multiomic Analysis of the UV-Induced DNA Damage Response.
    Boeing S, Williamson L, Encheva V, Gori I, Saunders RE, Instrell R, Aygün O, Rodriguez-Martinez M, Weems JC, Kelly GP, Conaway JW, Conaway RC, Stewart A, Howell M, Snijders AP, Svejstrup JQ.
    Cell Rep. 2016 S2211-1247
  • Inhibition of fatty acid desaturation is detrimental to cancer cell survival in metabolically compromised environments.
    Peck B, Schug ZT, Zhang Q, Dankworth B, Jones DT, Smethurst E, Patel R, Mason S, Jiang M, Saunders R, Howell M, Mitter R, Spencer-Dene B, Stamp G, McGarry L, James D, Shanks E, Aboagye E, Critchlow SE, Leung HY, Harris AL, Wakelam MJ, Gottlieb E, Schulze A
    Cancer Metab. 4:6
  • Knockout of the PKN Family of Rho Effector Kinases Reveals a Non-redundant Role for PKN2 in Developmental Mesoderm Expansion.
    Quétier I, Marshall JJ, Spencer-Dene B, Lachmann S, Casamassima A, Franco C, Escuin S, Worrall JT, Baskaran P, Rajeeve V, Howell M, Copp AJ, Stamp G, Rosewell I, Cutillas P, Gerhardt H, Parker PJ, Cameron AJ
    Cell Rep 14(3):440-8
  • The sorting protein PACS-2 promotes ErbB signalling by regulating recycling of the metalloproteinase ADAM17.
    Dombernowsky SL, Samsoe-Petersen J, Petersen CH, Instrell R, Hedegaard AM, Thomas L, Atkins KM, Auclair S, Albrechtsen R, Mygind KJ, Frohlich C, Howell M, Parker P, Thomas G, Kveiborg M.
  • Functional screening identifies MCT4 as a key regulator of breast cancer cell metabolism and survival.
    Baenke F, Dubuis S, Brault C, Weigelt B, Dankworth B, Griffiths B, Jiang M, Mackay A, Saunders B, Spencer-Dene B, Ros S, Stamp G, Reis-Filho JS, Howell M, Zamboni N, Schulze A.
    J Pathol. 2015 May 12. doi: 10.1002/path.4562.
  • High-throughput screening approaches to identify regulators of mammalian autophagy.
    Joachim J, Jiang M, McKnight NC, Howell M, Tooze SA.
    Methods. 2015 75:96-104.
  • Acetyl-CoA synthetase 2 promotes acetate utilization and maintains cancer cell growth under metabolic stress.
    Schug ZT, Peck B, Jones DT, Zhang Q, Grosskurth S, Alam IS, Goodwin LM, Smethurst E, Mason S, Blyth K, McGarry L, James D, Shanks E, Kalna G, Saunders RE, Jiang M, Howell M, Lassailly F, Thin MZ, Spencer-Dene B, Stamp G, van den Broek NJ, Mackay G, Bulusu
    Cancer Cell. 2015 27:57-71
  • Functional genomics identifies a requirement of pre-mRNA splicing factors for sister chromatid cohesion.
    Sundaramoorthy S, Vazquez-Novelle MD, Lekomtsev S, Howell M, Petronczki M.
    EMBO J. 2014 33(22):2623-42
  • Chromosomal instability selects gene copy-number variants encoding core regulators of proliferation in ER+ breast cancer.
    Endesfelder D, Burrell RA, Kanu N, McGranahan N, Howell M, Parker PJ, Downward J, Swanton C, Kschischo M.
    Cancer Res. 2014 74(17):4853-63.
  • Ccdc13 is a novel human centriolar satellite protein required for ciliogenesis and genome stability.
    Staples CJ, Myers KN, Beveridge RD, Patil AA, Howard AE, Barone G, Lee AJ, Swanton C, Howell M, Maslen S, Skehel JM, Boulton SJ, Collis SJ.
    J Cell Sci. 2014;127(Pt 13):2910-9.
  • Reduced NF1 expression confers resistance to EGFR inhibition in lung cancer.
    de Bruin EC, Cowell C, Warne PH, Jiang M, Saunders RE, Melnick MA, Gettinger S, Walther Z, Wurtz A, Heynen GJ, Heideman DA, Gomez-Roman J, Garcia-Castano A, Gong Y, Ladanyi M, Varmus H, Bernards R, Smit EF, Politi K, Downward J.
    Cancer Discov. 2014 4:606-19.
  • Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila.
    Wehr MC, Holder MV, Gailite I, Saunders RE, Maile TM, Ciirdaeva E, Instrell R, Jiang M, Howell M, Rossner MJ, Tapon N.
    Nature Cell Biology 2013:15:61-71
  • Affordable Luciferase Reporter Assay for Cell-Based High-Throughput Screening.
    Siebring-van Olst E, Vermeulen C, de Menezes RX, Howell M, Smit EF, van Beusechem VW.
    J Biomol Screen. 2012 Oct 30
  • The centriolar satellite protein Cep131 is important for genome stability.
    Staples CJ, Myers KN, Beveridge RD, Patil AA, Lee AJ, Swanton C, Howell M, Boulton SJ, Collis SJ.
    J Cell Sci. 2012 Jul 13.
  • Determination of synthetic lethal interactions in KRAS oncogene-dependent cancer cells reveals novel therapeutic targeting strategies.
    Steckel M, Molina-Arcas M, Weigelt B, Marani M, Warne PH, Kuznetsov H, Kelly G, Saunders B, Howell M, Downward J, Hancock DC.
    Cell Res. 2012 22:1227-45
  • Functional metabolic screen identifies 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 as an important regulator of prostate cancer cell survival.
    Ros S, Santos CR, Moco S, Baenke F, Kelly G, Howell M, Zamboni N, Schulze A.
    Cancer Discov. 2012 2:328-43
  • Binding of dynein intermediate chain 2 to paxillin controls focal adhesion dynamics and migration
    Rosse C, Boeckeler K, Linch M, Radtke S, Frith D, Barnouin K, Morsi AS, Hafezparast M, Howell M, Parker PJ.
    J Cell Sci. 2012125:3733-8
  • Genome-wide RNA interference analysis of renal carcinoma survival regulators identifies MCT4 as a Warburg effect metabolic target.
    Gerlinger M, Santos CR, Spencer-Dene B, Martinez P, Endesfelder D, Burrell RA, Vetter M, Jiang M, Saunders RE, Kelly G, Dykema K, Rioux-Leclercq N, Stamp G, Patard JJ, Larkin J, Howell M, Swanton C.
    J Pathol. 2012 227:146-56
  • Genome-wide siRNA screen reveals amino acid starvation-induced autophagy requires SCOC and WAC.
    McKnight NC, Jefferies HB, Alemu EA, Saunders RE, Howell M, Johansen T, Tooze SA.
    EMBO J. 2012 31:1931-46
  • Tales from an academic RNAi screening facility; FAQs.
    Jiang M, Instrell R, Saunders B, Berven H, Howell M.
    Brief Funct Genomics. 2011 10:227-37
  • Anomalous inhibition of c-Met by the kinesin inhibitor aurintricarboxylic acid
    Milanovic M, Radtke S, Peel N, Howell M, Carriere V, Joffre C, Kermorgant S, Parker PJ.
    Int J Cancer. 2012 130:1060-70
  • PKCa and PKCd regulate ADAM17-mediated ectodomain shedding of heparin binding-EGF through separate pathways.
    Kveiborg M, Instrell R, Rowlands C, Howell M, Parker PJ.
    PLoS One. 2011 6:e17168.
  • Chromosomal instability confers intrinsic multidrug resistance.
    Lee AJ, Endesfelder D, Rowan AJ, Walther A, Birkbak NJ, Futreal PA, Downward J, Szallasi Z, Tomlinson IP, Howell M, Kschischo M, Swanton C.
    Cancer Res. 2011 71:1858-70.
  • Combined functional genomic and proteomic approaches identify a PP2A complex as a negative regulator of Hippo signaling.
    Ribeiro PS, Josue F, Wepf A, Wehr MC, Rinner O, Kelly G, Tapon N, Gstaiger M.
    Mol Cell. 2010 39:521-34.
  • Predictive biomarker discovery through the parallel integration of clinical trial and functional genomics datasets.
    Swanton C, Larkin JM, Gerlinger M, Eklund AC, Howell M, Stamp G, Downward J, Gore M, Futreal PA, Escudier B, Andre F, Albiges L, Beuselinck B, Oudard S, Hoffmann J, Gyorffy B, Torrance CJ, Boehme KA, Volkmer H, Toschi L, Nicke B, Beck M, Szallasi Z.
    Genome Med. 2010 2:53.
  • Manipulating signal delivery - plasma-membrane ERK activation in aPKC-dependent migration
    Boeckeler K, Rosse C, Howell M, Parker PJ.
    J Cell Sci. 2010 123:2725-32
  • Nuclear import impairment causes cytoplasmic trans-activation response DNA-binding protein accumulation and is associated with frontotemporal lobar degeneration.
    Nishimura AL, Zupunski V, Troakes C, Kathe C, Fratta P, Howell M, Gallo JM, Hortobagyi T, Shaw CE, Rogelj B.
    Brain. 2010 133:1763-71
  • Chromosomal instability determines taxane response.
    Swanton C, Nicke B, Schuett M, Eklund AC, Ng C, Li Q, Hardcastle T, Lee A, Roy R, East P, Kschischo M, Endesfelder D, Wylie P, Kim SN, Chen JG, Howell M, Ried T, Habermann JK, Auer G, Brenton JD, Szallasi Z, Downward J.
    Proc Natl Acad Sci U S A. 2009 106:8671-6
  • siRNA screening of the kinome identifies ULK1 as a multi-domain modulator of autophagy.
    Chan EY, Kir S, Tooze SA.
    J Biol Chem. 2007 282:25464-74
  • Arkadia activates Smad3/Smad4-dependent transcription by triggering signal-induced SnoN degradation.
    Levy L, Howell M, Das D, Harkin S, Episkopou V, Hill CS.
    Mol Cell Biol. 2007 27:6068-83.
  • Regulators of mitotic arrest and ceramide metabolism are determinants of sensitivity to paclitaxel and other chemotherapeutic drugs.
    Swanton C, Marani M, Pardo O, Warne PH, Kelly G, Sahai E, Elustondo F, Chang J, Temple J, Ahmed AA, Brenton JD, Downward J, Nicke B.
    Cancer Cell 2007 11:498-512.