| Screen Status: | Primary |
| Screen Overview: | Genome screen of S2R cells with the Drosophila Ambion Library |
| Screener: | Michael Wehr (Tapon Lab). Extension:3661, Rm 230 |
| Library: | Ambion Genome Library |
| Library Description: | Drosophila genome library in 36 384-well plates from Ambion |
| Library Supplier: | Ambion |
| Library Format: | 36 plate(s) in 384 well format |
| Replicate Info: | 3 replicates |
| Publications: | Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila.. Wehr et al., 2012 |
| Keywords: | Hippo signalling |
| Cell Line: | S2R+ |
Day 1: Drosophila cell transfection
Drosophila S2R+ were transfected with DNA (Yki-NTEV, 14.3.3eps-CTEV, GV-2ER, UAS-Fluc and pIZ-Rluc) using FuGENEHD transfection reagent. The cells were then bathed with the dsRNA library (Ambion) and cultured at 25 °C, 0% CO2 for 3 days.
Day 4: Cell lysis
The culture media was aspirated and Passive Lysis Buffer was added.
Day 5: Dual-luciferase reporter assay
Firefly luciferse and Renilla luciferase activity were quantified on the Envision plate reader.