Protocol: |
Mix siRNA in 2.5uL HBSS to 375nM concentration. Mix 0.1uL Interferin in 2.5uL OPTIMEM. Mix together on plate and leave for 20 minutes. Add cells in 20ul/well. Top-up 25ul fresh media per well after 24hour. Culture the cells for further 48hours |
Delivery Reagent: |
Interferin |
Delivery Reagent Cat No: |
409-10 |
Delivery Reagent Supplier: |
Polyplus(via SourceBioscience) |
Delivery Reagent Batch No: |
051NF0204G6 |
Delivery Reagent Concentration: |
0.1ul/well |
Silencing Reagent Concentration: |
37.5nM (b.f. 24hour) |
Silencing Reagent Supplier: |
Dharmacon |
Silencing Reagent Origin: |
HTS Lab |