| Protocol: |
Mix siRNA in 2.5uL HBSS to 375nM concentration. Mix 0.05uL Dharmafect2 in 2.5uL OPTIMEM. Mix together on plate and leave for 20 minutes. Add cells in 20ul/well. After 24hrs, add 75ul fresh media, culture the cells for further 48hours. |
| Delivery Reagent: |
Dharmafect2 |
| Delivery Reagent Cat No: |
T-2002 |
| Delivery Reagent Supplier: |
Dharmacon |
| Delivery Reagent Batch No: |
111219T |
| Delivery Reagent Concentration: |
0.05ul/well |
| Silencing Reagent Concentration: |
37.5nM |
| Silencing Reagent Supplier: |
Dharmacon |
| Silencing Reagent Origin: |
HTS Lab |