| Protocol: |
Mix siRNA in 10uL HBSS to 37.5nM final concentration. Mix 0.1uL Genemute in 10uL OPTIMEM. Mix together on plate and leave for 20 minutes. Add cells in 80ul/well. Top-up the media with 100ul fresh media after 24hours, then culture the cells for further 72hours under normal/hypoxia condition |
| Delivery Reagent: |
Genemute |
| Delivery Reagent Cat No: |
SL10568 |
| Delivery Reagent Supplier: |
Tebu-Bio (Signa Gen) |
| Delivery Reagent Batch No: |
Lot268256 |
| Delivery Reagent Concentration: |
0.1ul/well |
| Silencing Reagent Concentration: |
37.5nM |
| Silencing Reagent Supplier: |
Dharmacon (On-target non-target plus) |
| Silencing Reagent Origin: |
Schulze Lab |