| Plate Format: | 96 well |
| Plate Coating: | Poly-L-Lysine |
| Transgene: | Mad2+/- |
| Origin: | Swanton Lab |
| Cell Medium: | E4+10%FCS+1%PenStrep |
| Cell Seeding Density Per Well: | 4000 |
| Protocol: | Mix siRNA in 10uL HBSS to 37.5nM final concentration. Mix 0.3uL Lipofectamine RNAiMAX in 10uL OPTIMEM. Mix together on plate and leave for 20 minutes. Add cells in 80ul/well. Culture the cells for further 72hours |
| Delivery Reagent: | Lipofectamine RNAiMAX |
| Delivery Reagent Cat No: | 13778500 |
| Delivery Reagent Supplier: | Invitrogen |
| Delivery Reagent Concentration: | 0.3ul/well |
| Silencing Reagent Concentration: | 37.5nM |
| Silencing Reagent Supplier: | Dharmacon |
| Silencing Reagent Origin: | HTS Lab |