| Plate Format: | 384 well |
| Origin: | Downward Lab |
| Cell Medium: | RPMI+10%FCS+1%PenStrep |
| Cell Seeding Density Per Well: | 500 |
| Protocol: | Mix miRNA mimic/inhibitor in 2.5uL HBSS to 375nM concentration. Mix 0.075uL Dharmafect2 in 3uL OPTIMEM. Mix together on plate and leave for 20 minutes. Add cells in 80ul/well. Culture the cells for 96hours. |
| Delivery Reagent: | Dharmafect2 |
| Delivery Reagent Cat No: | T-2002 |
| Delivery Reagent Supplier: | Dharmacon |
| Delivery Reagent Concentration: | 0.075ul/well |
| Silencing Reagent Concentration: | 11nM |
| Silencing Reagent Supplier: | Qiagen |
| Silencing Reagent Origin: | HTS Lab |