| Protocol: |
Mix siRNA in 2.5uL HBSS to 375nM concentration. Mix 0.05uL Interferin in 3uL OPTIMEM. Mix together on plate and leave for 20 minutes. Add cells in 40ul/well. Top-up 50ul fresh media with drug per well after 24hour. Culture the cells for further 72hours |
| Delivery Reagent: |
Lipofectamine RNAiMAX |
| Delivery Reagent Cat No: |
13778500 |
| Delivery Reagent Supplier: |
Invitrogen |
| Delivery Reagent Concentration: |
0.05ul/well |
| Silencing Reagent Concentration: |
18.75nM |
| Silencing Reagent Supplier: |
Dharmacon |
| Silencing Reagent Origin: |
HTS Lab |