Protocol: |
Mix siRNA in 10uL HBSS to 37.5nM final concentration. Mix 0.225uL Lullaby in 10uL OPTIMEM. Mix together on plate and leave for 20 minutes. Add cells in 80ul/well. Top-up 100ul fresh media after 48hours, then culture the cells for further 48hours |
Delivery Reagent: |
Lullaby |
Delivery Reagent Cat No: |
LL73000 |
Delivery Reagent Supplier: |
Oz Bioscience |
Delivery Reagent Batch No: |
LL101-214 |
Delivery Reagent Concentration: |
0.225ul/well |
Silencing Reagent Concentration: |
37.5nM |
Silencing Reagent Supplier: |
Dharmacon |
Silencing Reagent Origin: |
HTS Lab |