Day 1: Cells reverse transfected

8 breast cancer cell lines and 4 prostate cancer cell lines are transfected using Lullaby with custom designed on-target plus metabolic enzymes library (Dharmacon) for 1 day under 10% serum / 1% serum culture media.

Day 2: The transfected cells are topped up with extra fresh media and cultured under normal and hypoxia condition for further 3 days.

Day 5: The plates are fixed with 80% EthOH and stained with DAPI.

Readout

Acumen Explorer eX3 laser scanning cytometer was used to determine cell number per well.