|Screener:||Barrie Peck (Schulze Lab).
Extension:2049, Rm 113
|Library:||Custom Order - Barrie|
|Library Description:||Barrie's Custom Plate MCTMA 363141|
|Library Format:||1 plate(s) in 96 well format|
|Replicate Info:||3 replicates
|Publications:||Acetyl-CoA synthetase 2 promotes acetate utilization and maintains cancer cell growth under metabolic stress.. Schug et al., 2015
|Keywords:||Hypoxia, Metabolism, Normoxia, Viability|
Day 1: Cells reverse transfected
8 breast cancer cell lines and 4 prostate cancer cell lines are transfected using Lullaby with custom designed on-target plus metabolic enzymes library (Dharmacon) for 1 day under 10% serum / 1% serum culture media.
Day 2: The transfected cells are topped up with extra fresh media and cultured under normal and hypoxia condition for further 3 days.
Day 5: The plates are fixed with 80% EthOH and stained with DAPI.
Acumen Explorer eX3 laser scanning cytometer was used to determine cell number per well.