| Plate Format: | 96 well |
| Transgene: | AP-HBEGF |
| Origin: | Parker lab |
| Cell Medium: | DMEM + 10% FBS + 1% L-Glutamine |
| Cell Seeding Density Per Well: | 5000 |
| Protocol: | Mix siRNA in 10uL HBSS to 37.5nM final concentration. Mix 0.3uL INTERFERin in 10uL OPTIMEM. Mix together on plate and leave for 20 minutes. Add cells in 80ul/well. |
| Delivery Reagent: | INTERFERin |
| Delivery Reagent Cat No: | 409-50 |
| Delivery Reagent Supplier: | Polyplus |
| Delivery Reagent Batch No: | 03INF1011C |
| Delivery Reagent Concentration: | 0.3ul/well |
| Silencing Reagent Concentration: | 37.5nM |
| Silencing Reagent Supplier: | Dharmacon |
| Silencing Reagent Origin: | HTS lab |