Genome Screen Protocol

Day 1: Transfection

RPE1 cells were reverse transfected with siRNA (Dharmacon) using Lullaby transfection reagent in 384 well plates.

Day 3: Treatment with 3µM ICRF193, 1µM Nocodazole

Media containing ICRF and Nocodazole compounds were added on top of the culture media.

Day 4: Cell fixation

Plates were fixed with Ethanol/Acidic (9.5:0.5) and stored at -20C.

Day 5: Staining

Cells were stained with MPM2-Cy5 and Pacific Green antibodies.


Plates are read on ArrayScan microscope and the following measurements are taken:

Plates read on Acumen Explorer and following measurements taken: