Genome Screen Protocol
Day 1: Transfection
RPE1 cells were reverse transfected with siRNA (Dharmacon) using Lullaby transfection reagent in 384 well plates.
Day 3: Treatment with 3µM ICRF193, 1µM Nocodazole
Media containing ICRF and Nocodazole compounds were added on top of the culture media.
Day 4: Cell fixation
Plates were fixed with Ethanol/Acidic (9.5:0.5) and stored at -20C.
Day 5: Staining
Cells were stained with MPM2-Cy5 and Pacific Green antibodies.
Plates are read on ArrayScan microscope and the following measurements are taken:
Plates read on Acumen Explorer and following measurements taken: