Day 1: Transfection of PC9 cells

PC9 cells were transfected using Dharmafect2 transfection reagent and cultured for 2 days.

Day 3: Addition of Erlotinib

For the drug treated plates, the culture media was aspirated and fresh media containining Erlotinib was added (final concentration 30nM). For control plates, fresh media only was added. The plates were then cultured for a further 3 days.

Day 6: Fix the plates with 80% EthOH and stain the plates with DAPI

Plates were fixed with 80% Ethanol and stained with DAPI.

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